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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Bt okra
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OE-17A
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Organization:Maharashtra Hybrid Seeds Company Limited (Mahyco)Private sector (business and industry), Head OfficeJalna - Aurangabad Road, Post Box no. 76, Dawalwadi, JalnaDawalwadi, Maharashtra
431203, IndiaPhone: +91 02482-262471,Fax: +91 02482-26002,Email: info@mahyco.com,Website: https://mahyco.com/,
The okra (Abelmoschus esculentus) was modified for resistance to Lepidoptera pests (e.g. spotted bollworm) through the expression of Bacillus thuringiensis Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the okra contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-115629-1 Organism Abelmoschus esculentus (Okra, Lady's finger)Crops
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pC2300cry1Ac10518ve
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
The modified okra includes two gene cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase II (nptII).
The cry1Ac is under control of the Cauliflower mosaic virus (CaMV) 35S enhanced promoter and a poly(A) terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at high levels.
Transcription of nptII is under control of the CaMV 35S promoter and 35S terminator. Again, transcription is expected to occur at high levels due to the nature of the viral promoter.
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The cry1Ac is under control of the Cauliflower mosaic virus (CaMV) 35S enhanced promoter and a poly(A) terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at high levels.
Transcription of nptII is under control of the CaMV 35S promoter and 35S terminator. Again, transcription is expected to occur at high levels due to the nature of the viral promoter.
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- Food
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- Transformation vector.pdf [ English ]
- Description from Bt okra from provisional patent.pdf [ English ]
- Sequences - provisional patent.pdf [ English ]
- Efficient genetic transformation of okra (Abelmoschus esculentus (L.) Moench) and generation of insect-resistant transgenic plants expressing the cry1Ac gene.PDF [ English ]
